@article{Chularojmontri_Nanna_2022, title={Effect of Carallia brachiata Ethanolic Extract on Adipogenesis in 3T3-L1 Adipocytes}, volume={22}, url={https://asianmedjam.com/index.php/amjam/article/view/1305}, DOI={10.14456/2022s10733}, abstractNote={<p><strong>Introduction:</strong> An excessstorage of body fat causes obesity. Since obesity increasesrisk of chronic diseases, thusit isimportant to inhibit excessive storage of fat. <em>Carallia brachiata</em> is a plant that found in Thailand. There is a report that the leaf of <em>Carallia brachiata</em> has an antidiabetic effect in rat model.</p> <p><strong>Objectives:</strong> The aim of this study was to demonstrate the effect of <em>Carallia brachiata</em> leaf and stem ethanolic extracts (CL and CS) on adipogenesis in 3T3-L1 adipocytes.</p> <p><strong>Methods:</strong> 3T3-L1 adipocytes were used for measuring cytotoxicity of CL and CS (3, 10, 30, and 100 μg/mL). To determine adipogenesis process, CL and CS extracts were added to the cell culture medium at concentrations of 0, 3, 10, 30 and 100 μg/mL. After 8 days of treatments, adipocyte cells were stained with Oil Red O solution and measured the expression of adipogenic genes.</p> <p><strong>Results:</strong> CL and CS did not inhibit cell proliferation and showed no cytotoxicity in 3T3-L1 cells. Therefore, concentration range of 3 - 100 μg/mL CL and CS was used for subsequent experiments. CS inhibited lipid accumulation in 3T3-L1 adipocytes and suppressed gene<br />expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein 1c (SREBP1c), adipocyte-specific genes fatty acid synthase (FAS), lipoprotein lipase (LPL) and adipocyte fatty acid-binding protein 2 (aP2). Whereas, CL dis not inhibit lipid storage and only had an inhibitory activity on SREBP1c and aP2 genes.</p> <p><strong>Conclusions:</strong> Our findings suggest that <em>Carallia brachiata</em> has an inhibitory effect on adipogenesis could be partially caused by suppressing C/EBPα, PPARγ and SREBP1c genes in 3T3-L1 adipocytes</p>}, number={-}, journal={Asian Medical Journal and Alternative Medicine}, author={Chularojmontri, Linda and Nanna, Urarat}, year={2022}, month={Aug.}, pages={S126} }