Bio-extract Concentrated of Thai "Yor" Morinda citrifolia Effects in Analgesic, Acute Toxicity and Human Peripheral Blood Mononuclear Cells

Abstract

BCTX, Bio-extract concentrated Thai "Yor" or Noni in English, Morinda citrifolia L. (Rubiaceae),was found the total fatty acids and amino acids content of 149 ± 8.74 and 204 mg/100 g,respectively. It was reported to be a nontoxic bio-extract with the acute toxicity (LD50) of morethan 4,000 mg/kg body weight of Swiss albino mice and Wistar rat. The methyl alcohol extractof M. citrifolia raw material from BCTY product revealed the analgesic effect of 93.1 ± 1.7%inhibition which was quite equivalent to 96.7 ± 1.6% of morphine sulfate 1.5 mg/kg. Human peripheralblood mononuclear cells (HPBMCs) were used for cytotoxicity, CD₄/CD₈ counts. The BCTY extract500 μg/ml increased in cell viability 160.29 ± 1.35% (compared to 100% of the control) andstimulated lymphocyte proliferation 84.04 ± 10.42% while the CD₄ and CD₈ were 107.83 ± 3.21%and 98.00 ± 2.74%, respectively.

Key words: Yor, Noni, M. citrifolia L. (Rubiaceae), immune activity, nontoxic bio-extract, humanperipheral blood mononuclear cells (HPBMCs), cytotoxicity