In Vitro α-Amylase, α-Glucosidase Inhibition, and Anti-Oxidant Activities of Plant Components in Ya-Hom Teppajid
Keywords:Ya-Hom Teppajid, α-amylase, α-glucosidase, Diabetes mellitus, Antioxidant
Introduction: Diabetes is characterized by high blood sugar levels or hyperglycemia causing the increase of free radicals which increase the risk of various diseases. Ya-Hom Teppajid which consists of 45 plants and their properties had shown anti-diabetic activities in some herbs and the rest of the herbs have not been studied for these activities.
Methods: The ethanolic extract of plant componentsinYa-Hom Teppajid were to investigate α-amylase, α-glucosidase inhibitory, and antioxidant activities (DPPH, and TBARS), including total phenolic (TPC) and total flavonoid (TFC).
Results: Twenty-four plants showed antioxidant activities, while fifteen plants exhibited enzyme inhibition of diabetes mechanisms. The ethanolic extract of N. lotus had stronger enzyme inhibitory activities and antioxidant activities than other plant components, although less than the positive standard.
Conclusions: Our result can support the efficacy of Ya-Hom-Teppajid for a diabetic. Interestingly, the whole flower of N. lotus was suggested as a better part due to its stronger antioxidant activity by free radicals scavenging property. These herbs can be consumed as food ingredients or food supplements and their consumption should be beneficial to diabetic patients.
Saeedi P, Petersohn I, Salpea P, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045:
Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract. 2019;157:107843.
International Diabetes Federation. IDF Diabetes Atlas - 8th Edition. International Diabetes Federation: 2017.
Cordonnier DJ, Zaoui P, Halimi S. Role ofACE inhibitors in patients with diabetes mellitus. Drugs. 2001;61(13):1883-1892.
Kazeem MI, Adamson JO, Ogunwande IA. Modes of inhibition of α -amylase and α -glucosidase by aqueous extract of Morinda lucida Benth leaf. Biomed Res Int. 2013;2013:527570.
Bras NF, Diogo SM, Pedro A, Fernandes, Maria J. Mechanistic Pathway on Human α-Glucosidase Maltase-Glucoamylase Unveiled by QM/MM Calculations. J Phys Chem B. 2018;122(14):3889-3899.
Rajendran A, Sudeshraj R, Sureshkumar S. Potential antidiabetic activity of medicinal plants - A short review. Int J Phytopharm. 2018;7(5):456-459.
Matsuzaki S, Szweda PA, Szweda LI, Humphries KM. Regulated production of free radicals by the mitochondrial electron transport chain: Cardiac ischemic preconditioning. Adv Drug Deliv Rev. 2009;61(14):1324-1331.
Ministry of Public Health. National List of Essential Medicines of Thailand. Ministry of Public Health. http://kpo.moph.go.th/webkpo/tool/Thaimed2555.pdf. Published 2013. Accessed May 6, 2022.
Alam MA, Subhan N, Rahman MM, Uddin SJ, Reza HM, Sarker SD. Effect of citrus flavonoids,naringinandnaringenin,onmetabolic syndrome and their mechanisms of action. Adv Nutr. 2014;5(4):404-417.
Boonpisuttinant K, Winitchai S,Akkarakultron P, Udompong S. In vitro alpha-glucosidase and alpha-amylase enzyme-inhibited activities by water-lily (Nymphaea genus) extracts. Malays Appl Biol. 2019;48(4):121-127.
Parimala M, Shoba FG. Evaluation of antidiabetic potential of Nymphaea nouchali burm. F. seeds in STZ - Induced diabetic rats. Int J Pharm Pharm Sci. 2014;6(4):536-541.
Dhandapani S, Subramanian VR, Rajagopal S, Namasivayam N. Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol Res. 2002;46(3):251-255.
Sathya S, Amarasinghe NR, Jayasinghe L, Araya H, Fujimoto Y. Enzyme inhibitors from the aril of Myristica fragrans. S Afr J Bot. 2020;130:172-176.
Thitikornpong W, Palanuvej C, Ruangrungsi N. In vitro antidiabetic, antioxidation and cytotoxicity activities of ethanolic extract
of Aquilaria crassna leaves and its active compound; mangiferin. Indian J Tradit Knowl. 2019;18(1):144-150.
Kooti W, Farokhipour M, Asadzadeh Z, Ashtary-Larky D, Asadi-Samani M. The role of medicinal plants in the treatment of diabetes: a systematic review. Electron Physician. 2016;8(1):1832-1842.
Ranasinghe P, Perera S, Gunatilake M, et al. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model. Pharmacognosy Res. 2012;4(2):73-79.
Parmar HS, Kar A. Medicinal values of fruit peels from Citrus sinensis, Punica granatum, and Musa paradisiaca with respect to alterations in tissue lipid peroxidation and serum concentration of glucose, insulin, and thyroid hormones. J Med Food. 2008;11(2):376-381.
Ademiluyi AO, Oboh G. Soybean phenolicrich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro. Exp Toxicol Pathol. 2013;65(3):305-309.
Gowri PM, Tiwari AK, Ali AZ, Rao JM. Inhibition of alpha-glucosidase and amylase by bartogenic acid isolated from Barringtonia racemosa Roxb. seeds. Phytother Res. 2007; 21(8):796-799.
Yamasaki K, Hashimoto A, Kokusenya Y, Miyamoto T, Sato T. Electrochemical method for estimating the antioxidative effects of methanol extracts of crude drugs. J Chem Pharm Bull (Tokyo). 1994;42(8):1633-1665.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-358.
Folin O, Ciocalteu V. On tyrosine and tryptophane determinations in proteins. J Biol Chem. 1927;73(2):627-650.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contentsin mulberry and their scavenging effects on superoxides radicals. J Food Chem. 1999;64(4):555-559.
Semaming Y, Chunpricha S, Suriya A. Antioxidant activity and protective effect against oxidative stress induced-hemolysis of Nymphaea lotus L. extracts. Asia Pac J Sci Technol. 2018;23(4):1-7.
Sreenathkumar S, Arcot S. Antidiabetic activity of Nymphaea pubescens Willd - a plant drug of aquatic flora interest. J Pharm Res. 2010;3(12):3067-3069.
Dhandapani S, Rajgopal VR, Rajagopal S, Namasivayam N. Hypolipidemic of effect Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol Res. 2002;46(3):251-255.
How to Cite
Copyright (c) 2022 Asian Medical Journal and Alternative Medicine
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.