In Vitro Anticancer and Anti-inflammatory Activities of Rauwenhoffia siamensis Scheff Root Extracts
Keywords:Rauwenhoffia siamensis, Alternative medicine, Herb, Endometrial cancer, Gynecologic cancer
Introduction: The sixth most prevalent malignancy among women is endometrial cancer. R. siamensis roots were utilized to treat endometrial cancer in Thai traditional medicine. However, the scientific basis for its action is not entirely understood.
Objectives: To investigate the cytotoxicity of R. siamensis root extracts against uterine, ovarian, and cervical cancer cells, anti-inflammatory property and their flavonoid contents.
Methods: Cytotoxicity activity was determined by using sulforhodamine B assay. Anti-inflammatory activity was investigated by inhibition of nitric oxide production. Flavonoid content was analysed using aluminum chloride colorimetric assay.
Results: Observations showed high flavonoid contents in all R. siamensis root extracts. Strong cytotoxic activity was detected in the ethanolic extract of R. siamensis root as well as production inhibition of nitric oxide.
Conclusions: The ethanolic extract of R. siamense showed the significant anticancer activity. To better comprehend its mechanism, active substances should be examined for cytotoxicity, and an animal model should be investigated.
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