Correlation of Angiogenic Biomarkers to Tumor Progression and Angiogenesis in Cervical Cancer Cell (CaSki)-implanted Nude Mice

Authors

  • Nakorn Mathuradavong Division of Physiology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Umarat Srisawat Division of Physiology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Bhornprom Yoysungnoen Division of Physiology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Nattapon Sookprasert Division of Physiology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand

DOI:

https://doi.org/10.14456/2022030105

Keywords:

Angiogenic biomarkers, Cervical cancer, CaSki, Angiogenesis, Tumor progression

Abstract

Introduction: Several studies have indicated that microvascular density (MVD) and the expressions of angiogenic biomarkers were associated with tumor growth and angiogenesis in cervical cancer. However, the results were incomplete and inconsistent.

Objectives: To determine the correlation of the angiogenic biomarkers, including vascular endothelial growth factor (VEGF), hypoxia-inducible factor 1-alpha (HIF-1α), cyclooxygenase-2 (COX-2), and epidermal growth factor receptor (EGFR), to tumor progression and angiogenesis in cervical cancer cell (CaSki)-implanted nude mice model.

Methods: 10×106 of CaSki cells were injected into the female nude mice (n = 50) to establish subcutaneous tumors. The tumor size was measured every 3 days for one month. The MVD was evaluated using the CD31 expression. VEGF, HIF-1α, COX-2, and EGFR expression
were detected by immunohistochemistry.

Results: The results showed that HIF-1α (r = 0.979 and r = 0.942), VEGF (r = 0.972 and r = 0.929), COX-2 (r = 0.982 and r = 0.957), and EGFR (r = 0.993 and r = 0.971) closely correlated with tumor growth and tumor angiogenesis, respectively. Interestingly, EGFR was mostly involved in tumor growth and angiogenesis.

Conclusions: This study demonstrates that the HIF-1α, VEGF, COX-2, and EGFR are a set of biological markers which are strongly related to tumor progression and angiogenesis in cervical cancer.

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Published

2022-12-28

How to Cite

[1]
Mathuradavong, N., Srisawat, U., Yoysungnoen, B. and Sookprasert, N. 2022. Correlation of Angiogenic Biomarkers to Tumor Progression and Angiogenesis in Cervical Cancer Cell (CaSki)-implanted Nude Mice. Asian Medical Journal and Alternative Medicine. 22, 3 (Dec. 2022), 202–211. DOI:https://doi.org/10.14456/2022030105.

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Original Articles