Molecular Cloning and Characterization of a Novel Transforming Growth Factor-β Homologue (TGH) Derived from Trichinella spiralis

Authors

  • Salisa Chaimon Research Unit in Nutraceuticals and Food Safety, Thammasat University, Pathum Thani, Thailand
  • Poom Adisakwattana Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
  • Pathanin Chantree Division of Anatomy, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Pongsakorn Martviset Division of Parasitology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand

DOI:

https://doi.org/10.14456/2022s10711

Keywords:

Trichinella spiralis, Transforming growth factor β (TGF-β), TGF-β homologue (TGH), Immunomodulation

Abstract

Introduction: Transforming growth factor-β (TGF- β) is a pleiotropic cytokine that particularly involves in cell biology and regulates immune responses. Recently, TGF-β homologues (TGHs) have been identified in several organisms, including parasitic helminths. Previous studies suggested that TGHs derived from filaria and Fasciola regulate parasite development and differentiation as well as immunomodulation. In Trichinella spiralis, there are several TGH isoforms reported in its genome but the properties, especially, immunomodulation have not been characterized.

Objectives: This study aimed to identify and characterize the molecular properties and biochemical functions of TGH from T. spiralis (TsTGH2)

Methods: Full-length TsTGH2 was obtained from the genome of T. spiralis and characterized by using bioinformaticstools. Recombinant TsTGH2 (rTsTGH2) was expressed in BL21(DE3) E. coli. The native TsTGH2 was detected in the parasite extracts by Western analysis and localized in the parasite tissue by immunohistochemistry using rTsTGH2-specific antibodies. The interaction between TsTGH2 and human TGF-β receptor was investigated using TGF-β reporter cell line and the downstream molecules, phosphorylated-SMADs, were subsequently determined by using Western analysis.

Results: The bioinformatics analysis revealed that TsTGH2 contains nine cysteine residues that are conserved to TGF-β family. rTsTGH2 was successfully expressed in bacterial expression system and used for producing of polyclonal antibodies in mice. Native TsTGH2 could be detected in crude worm extract and parasite E/S products, and localized mainly in stichocytes and muscle of muscle-stage larvae. Furthermore, rTsTGH2 specifically bound to TGF-β receptor and mediated downstream signaling cascade through SMAD2/3 dependent pathway.

Conclusions: Our results suggested that TsTGH2 may be a key molecule involving in parasite development as well as host-parasite interaction by immunomodulation during their life cycle.

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Published

2022-08-01

How to Cite

[1]
Chaimon, S., Adisakwattana, P., Chantree, P. and Martviset, P. 2022. Molecular Cloning and Characterization of a Novel Transforming Growth Factor-β Homologue (TGH) Derived from Trichinella spiralis. Asian Medical Journal and Alternative Medicine. 22, - (Aug. 2022), S102. DOI:https://doi.org/10.14456/2022s10711.