The Expression of Alkaline Phosphatase in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells

Authors

  • Nuengruethai Chadee Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Jintamai Suwanprateep Biomedical Engineering Research Unit, National Metal and Materials Technology Center (MTEC), Ministry of Science and Technology, Pathum Thani, Thailand
  • Chairat Tantrawatpan Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Pakpoom Kheolamai Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Duangrat Tantikanlayaporn Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
  • Sirikul Manochantr Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand

DOI:

https://doi.org/10.14456/2022s10719

Keywords:

Mesenchymal stem cells, Metformin, Osteogenic differentiation, Umbilical cord, Alkaline phosphatase

Abstract

Introduction: Mesenchymal stem cells (MSCs) have gained more interest in regenerative medicine. The umbilical cord, the alternative source of MSCs, has thus been increasingly recognized as a good MSC source for bone tissue regeneration and bone defect repair. Metformin, an anti-hyperglycemic drug, has been reported that promotes the osteogenic differentiation of BM-MSCs.
Objectives: This study aims to investigate the viability and the expression of alkaline phosphatasein metformin-treated umbilical cord-derived MSCs (UC-MSCs) during osteogenicdifferentiation.
Methods: The UC-MSCs were obtained from pregnant women after normal delivery. After charac-terization, UC-MSCs were cultured with different metformin concentrations (0 - 160 μM). The cell viability and alkaline phosphatase (ALP) expression were observed by MTT and alkaline phosphatase activity assay.
Results: The result showed that metformin at a concentration of 0 - 160 μM did not decrease the viability of UC-MSCs. Interestingly, the alkaline phosphatase activity of metformin-treated UC-MSCs was significantly increased, compared with untreated UC-MSCs in a dose-dependent manner.
Conclusions: The data suggested that metformin could enhance the expression of alkaline phosphatase and the osteogenic differentiation ability of MSCs derived from the umbilical cord.

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Published

2022-08-01

How to Cite

[1]
Chadee, N. , Suwanprateep, J. , Tantrawatpan, C. , Kheolamai, P. , Tantikanlayaporn, D. and Manochantr, S. 2022. The Expression of Alkaline Phosphatase in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells. Asian Medical Journal and Alternative Medicine. 22, - (Aug. 2022), S110. DOI:https://doi.org/10.14456/2022s10719.

Issue

Vol. 22 No. - (2022): Supplemental August 2022

Section

Abstracts

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