Quality Control, Antidiabetic, and Anti-inflammatory Effects, as Measured by Alpha-Amylase and Alpha-Glucosidase Activities, of a Weight-Loss Remedy from Worayokasan Scripture
Effect of a Weight-Loss Remedy
Keywords:Weight-Loss remedy, Alpha-Amylase, Alpha-Glucosidase, Type 2 diabetes
Introduction: Diabetes is characterized by chronic hyperglycemia, which increases the risk of free radical generation and disease. Obesity is a major risk factor for Type 2 Diabetes (T2DM). The Weight-Loss (WL) remedy from Worayokasan scripture, consisting of C. rotundus rhizomes (CR), T. chebula fruits (TCb), and T. crispa stem (TCp), has shown promising results for weight loss. However, there are no reports on its anti-diabetic activities.
Objectives: The objectives of this study were to analyze the quality control, anti-diabetic, and anti-inflammatory properties of the WL remedy.
Methods: Quality control was performed following the Thai Herbal Pharmacopoeia (THP) method. The anti-diabetic effects were measured by inhibition of α-amylase and α-glucosidase, and the anti-inflammatory effects were measured by reducing nitric oxide (NO) production of ethanolic and water extracts of the WL remedy and its plant constituents were also studied.
Results: The WL remedy passed the THP guidelines for quality control. T. crispa stem’ water extract (TCpW) was the most effective α-glucosidase inhibitor, with IC50 values of 201.28 µg/mL, outperforming the positive control acarbose (IC50 = 219.94 µg/mL). WLH, WLW, and WLE showed moderate α-glucosidase inhibitory activity, with IC50 values of 540.04, 680.07, and 894.32 µg/mL, respectively. However, all samples had low α-amylase inhibitory activity (IC50 > 1000 µg/mL). C. rotundus rhizomes' ethanolic extract (CRE) showed inhibited NO production with an IC50 value of 31.93 µg/mL.
Conclusions: The results of this study suggest that the WL remedy has anti-diabetic and weight-loss properties. It can be developed into a dietary supplement that may be beneficial for individuals with T2DM.
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