In Vitro Anti-oxidation, Anti-inflammatory and Anti-aging activities of Oak Extract
Keywords:
Anti-aging, Oak extract, Anti-oxidation, Anti-inflammatoryAbstract
Introduction: Intrinsic and extrinsic factors can influence skin aging. Intrinsic skin aging can occur due to the formation and accumulation of reactive oxygen species, furthermore UV radiation contributes up to 80% of extrinsic aging. Anti-oxidation bioactive components from natural sources have been recommended for skin aging prevention.
Objectives: we explored the anti-oxidation, anti-inflammatory and anti-aging activities of oak extract (freeze-dried) product.
Methods: Anti-oxidation activity was determined using superoxide dismutase (SOD) activity assay and radical scavenging activity assay (DPPH). Anti-inflammatory activity was determined by hyaluronidase activity inhibitory assay and hexosaminidase release inhibitory assay. Anti-aging activity was determined using inhibitory assay of human neutrophil elastase activity, inhibitory assay of matrix metalloproteinase-1 (MMP-1) and promoting assay of hyaluronic acid production.
Results: Anti-oxidation activity was observed for oak extract in both SOD activity assay and radical scavenging activity assay with IC50 of 2.27µg/mL and 3.25 µg/mL respectively. Anti-inflammatory activity was detected in hyaluronidase activity inhibitory assay with an IC50 of >400µg/ml and hexosaminidase release inhibitory effect was observed with IC50 of 223.4 µg/mL. The oak extract also exhibited anti-aging activity with human neutrophil elastase activity inhibitory at an IC50 of 20.87µg/mL and MMP-1 activity inhibitory effect at an IC50 of 125.7µg/mL. Oak extract did not promote any hyaluronic acid production in epidermal keratinocytes.
Conclusions: Oak extract exhibits a strong antioxidant effect comparable to control Baicalin and ascorbic acid. Together with the inhibitory effect on human neutrophil elastase and MMP-1, these results suggest that oak extract is a promising agent as an anti-aging material.
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