Unveiling the Botanical Riches: Enhancing Quality Control and Stability Assessment of Sa-Tri-Lhung-Klod Remedy Extract Through HPLC Profiling and Anti-inflammatory Potency Evaluation "
Keywords:
Sa-Tri-Lhung-Klod remedy, quality control, stability, HPLC fingerprint, anti-inflammatory activityAbstract
Introduction: Sa-Tri-Lhung-Klod remedy (ST) is a Thai traditional medicine preparation in the Thai National Drug List of Herbal Medicine, used for postpartum care such as excreting amniotic fluid, reducing inflammation, as a blood tonic, and improving blood circulation. Quality control and stability testing of this remedy are necessary to determine the standard quality of herbal formulations. In addition, the standard quality values and the stability of ST have not been reported.
Objectives: To assess the quality control and stability of the Sa-Tri-Lhung-Klod remedy.
Methods: The quality assessment of the ST and its plant ingredients was performed by moisture content, total ash, acid insoluble ash, extractive value, and tannin content, following the Thai Herbal Pharmacopoeia (THP) method. The stability testing of the ST extracts was stored under accelerated conditions for six months and was also determined by a chemical fingerprint study using HPLC and tested for anti-inflammatory activity on nitric oxide (NO) inhibitory in RAW264.7 cell lines.
Results: The quality of all plant materials passed the standard values of THP. The result of tannin content in the ST was increased upon extraction, especially the aqueous extraction. The stability under accelerated conditions of ST extracts showed that seven major constituents of the ethanolic extract (STE) were stable from day 0 to day 180. Moreover, STE has a stable anti-inflammatory effect in inhibiting nitric oxide production. While Brazilin, a constituent of the aqueous extract (STW) was unstable and undetectable from day 30 onwards.
Conclusions: STE was stable by HPLC fingerprint detection and exhibited anti-inflammatory activity within two years. However, STW should be stored in the refrigerator to prolong its shelf life.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Asian Medical Journal and Alternative Medicine
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.